A5213
Sigma
Monoclonal Anti-β-Amyloid antibody produced in mouse
clone BAM-10, ascites fluid
| Synonym: | Anti-A-BETA, Anti-Amyloid β Precursor Protein |
| MDL number: | MFCD00803867 |
Description
| Immunogen | synthetic β-amyloid peptide (1-40), conjugated to KLH. |
| Specificity | The antibody is useful in immunohistochemistry, immunoblotting,1 ELISA, and competitive ELISA. Also, this antibody has been used to neutralize Aβ assemblies in brains of transgenic mice expressing a mutant form of amyloid precursor protein,2 and for in vivo deep tissue imaging using near-IR optical spectrum.3 |
| The antibody reacts specifically with β-amyloid protein. The epitope recognized by the antibody resides within amino acids 1-12 of the β-amyloid protein. It specifically stains amyloid plaques within the cortex and amyloid deposits in blood vessels using formic acid-treated, formalin-fixed, paraffin-embedded, and Methacarn-fixed sections of human Alzheimer's disease (AD) brain tissue. |
Properties
| antibody form | ascites fluid |
| clone | BAM-10 (monoclonal) |
| contains | 15 mM sodium azide |
| species reactivity | human |
| application(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:2,000 using formic acid-treated, formalin-fixed, human Alzheimer′s disease (AD) brain sections. |
| indirect ELISA: suitable | |
| isotype | IgG1 |
| Gene Information | human ... APP(351) |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
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| A8354, Monoclonal Anti-β-Amyloid antibody produced in mouse | |
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References
| Cited Reference | 1. Tan, J., et al., Role of CD40 ligand in amyloidosis in transgenic Alzheimer's mice. Nat. Neurosci. 5, 1288-1293, (2002) |
| 2. Kotilinek, L.A., et al., Reversible memory loss in a mouse transgenic model of Alzheimer's disease. J. Neurosci. 22, 6331-63335, (2002) | |
| 3. Skoch, J., et al., Development of an optical approach for noninvasive imaging of Alzheimer's disease pathology. J. Biomed. Opt. 10, 11007, (2005) | |
| reference | Bing, G., et al., Localization of β-secretase memapsin 2 in the brain of Alzheimer’s patients and normal aged controls. Exp. Neurol. 175, 10-22, (2002) Abstract |
